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shh  (R&D Systems)


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    Structured Review

    R&D Systems shh
    Shh, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+sonic+hedgehog+shh+protein/Recombinant+Human+Sonic+Hedgehog%2FShh+Protein%2C+High+Activity/bio_rxiv__64898__2026__02__20__707051-217-14-15
    Average 95 stars, based on 38 article reviews
    shh - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Affinity Purification:

    Article Title: Induced hepatic stem cells are suitable for human hepatocyte production.
    Article Snippet: Penicillin-Streptomycin Solution (3100) FUJIFILM Wako Pure Chemical Corporation Cat# 16823191 ICG Sigma-Aldrich Cat# 155020 D-GaIN FUJIFILM Wako Pure Chemical Corporation Cat# 07505013 EzRIPA Lysis kit ATTO Cat# WSE-7420 EzApply ATTO Cat# 2332330 EzStandard PrestainBlue ATTO Cat# 2332347 c-PAGEL 10% ATTO Cat# 2331955 EzRun ATTO Cat# 2332310 P plus membranes ATTO Cat# 2322451 Filter paper ATTO Cat# 2392393 EzFastBlot ATTO Cat# 2332590 EzBlock Chemi ATTO Cat# 2332615 EzTBS ATTO Cat# 2332625 EzWestBlue ATTO Cat# 2332456 Precision Plus Protein All Blue Prestained Protein Standards Bio-Rad Laboratories, Inc. Cat# 1610373 Hanks’ Balanced Salt Solution (HBSS) Life Technologies Cat# 14025092 Critical commercial assays RNeasy Mini kit Qiagen N.V. Cat# 74106 SuperPREP II Cell Lysis & RT Kit for quantitative PCR TOYOBO CO., LTD. Cat# SCQ-401 FastStart Essential DNA Green Master Roche Cat# 06402712001 Luna Universal qPCR Master Mix New England Biolabs Inc. Cat# M3003E TaqMan Array 96-Well FAST Plate(Human Stem Cell Pluripotency) Applied Biosystems Cat# 4418722 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444963 Proteome Profiler Human Pluripotent Stem Cell Array Kit R&D Systems Cat# ARY010 PAS staining kit Muto Pure Chemicals Cat# 15792 Experimental models: Cell lines Cryopreserved Hepatocytes Species:Human, Lot#S1412T, Lot#S1238 and Lot#S1350 KaLy-Cell Cat# HHCPC-2M hiPSC lines 201B7 CiRA Foundation N/A hiPSC lines 15M66 CiRA Foundation N/A MEF cells ReproCELL Inc. Cat# RCHEFC003 Oligonucleotides oligonucleotides sequences were listed in Table S1 – – Software and algorithms US National Library of Medicine National Institutes of Health website National Institutes of Health (NIH) RRID:SCR_011446 https://www.ncbi.nlm.nih. gov/pubmed/ Primer 3 Plus application Primer 3 Plus RRID:SCR_003081 http://www.bioinformatics. nl/cgi-bin/primer3plus/pimer 3 plus.cgi R software program R Development Core Team RRID:SCR_001905 https://www.r-project.org (Continued on next page) 16 iScience 25, 105052, October 21, 2022

    Recombinant:

    Article Title: Induced hepatic stem cells are suitable for human hepatocyte production.
    Article Snippet: Penicillin-Streptomycin Solution (3100) FUJIFILM Wako Pure Chemical Corporation Cat# 16823191 ICG Sigma-Aldrich Cat# 155020 D-GaIN FUJIFILM Wako Pure Chemical Corporation Cat# 07505013 EzRIPA Lysis kit ATTO Cat# WSE-7420 EzApply ATTO Cat# 2332330 EzStandard PrestainBlue ATTO Cat# 2332347 c-PAGEL 10% ATTO Cat# 2331955 EzRun ATTO Cat# 2332310 P plus membranes ATTO Cat# 2322451 Filter paper ATTO Cat# 2392393 EzFastBlot ATTO Cat# 2332590 EzBlock Chemi ATTO Cat# 2332615 EzTBS ATTO Cat# 2332625 EzWestBlue ATTO Cat# 2332456 Precision Plus Protein All Blue Prestained Protein Standards Bio-Rad Laboratories, Inc. Cat# 1610373 Hanks’ Balanced Salt Solution (HBSS) Life Technologies Cat# 14025092 Critical commercial assays RNeasy Mini kit Qiagen N.V. Cat# 74106 SuperPREP II Cell Lysis & RT Kit for quantitative PCR TOYOBO CO., LTD. Cat# SCQ-401 FastStart Essential DNA Green Master Roche Cat# 06402712001 Luna Universal qPCR Master Mix New England Biolabs Inc. Cat# M3003E TaqMan Array 96-Well FAST Plate(Human Stem Cell Pluripotency) Applied Biosystems Cat# 4418722 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444963 Proteome Profiler Human Pluripotent Stem Cell Array Kit R&D Systems Cat# ARY010 PAS staining kit Muto Pure Chemicals Cat# 15792 Experimental models: Cell lines Cryopreserved Hepatocytes Species:Human, Lot#S1412T, Lot#S1238 and Lot#S1350 KaLy-Cell Cat# HHCPC-2M hiPSC lines 201B7 CiRA Foundation N/A hiPSC lines 15M66 CiRA Foundation N/A MEF cells ReproCELL Inc. Cat# RCHEFC003 Oligonucleotides oligonucleotides sequences were listed in Table S1 – – Software and algorithms US National Library of Medicine National Institutes of Health website National Institutes of Health (NIH) RRID:SCR_011446 https://www.ncbi.nlm.nih. gov/pubmed/ Primer 3 Plus application Primer 3 Plus RRID:SCR_003081 http://www.bioinformatics. nl/cgi-bin/primer3plus/pimer 3 plus.cgi R software program R Development Core Team RRID:SCR_001905 https://www.r-project.org (Continued on next page) 16 iScience 25, 105052, October 21, 2022

    Activity Assay:

    Article Title: Induced hepatic stem cells are suitable for human hepatocyte production.
    Article Snippet: Penicillin-Streptomycin Solution (3100) FUJIFILM Wako Pure Chemical Corporation Cat# 16823191 ICG Sigma-Aldrich Cat# 155020 D-GaIN FUJIFILM Wako Pure Chemical Corporation Cat# 07505013 EzRIPA Lysis kit ATTO Cat# WSE-7420 EzApply ATTO Cat# 2332330 EzStandard PrestainBlue ATTO Cat# 2332347 c-PAGEL 10% ATTO Cat# 2331955 EzRun ATTO Cat# 2332310 P plus membranes ATTO Cat# 2322451 Filter paper ATTO Cat# 2392393 EzFastBlot ATTO Cat# 2332590 EzBlock Chemi ATTO Cat# 2332615 EzTBS ATTO Cat# 2332625 EzWestBlue ATTO Cat# 2332456 Precision Plus Protein All Blue Prestained Protein Standards Bio-Rad Laboratories, Inc. Cat# 1610373 Hanks’ Balanced Salt Solution (HBSS) Life Technologies Cat# 14025092 Critical commercial assays RNeasy Mini kit Qiagen N.V. Cat# 74106 SuperPREP II Cell Lysis & RT Kit for quantitative PCR TOYOBO CO., LTD. Cat# SCQ-401 FastStart Essential DNA Green Master Roche Cat# 06402712001 Luna Universal qPCR Master Mix New England Biolabs Inc. Cat# M3003E TaqMan Array 96-Well FAST Plate(Human Stem Cell Pluripotency) Applied Biosystems Cat# 4418722 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444963 Proteome Profiler Human Pluripotent Stem Cell Array Kit R&D Systems Cat# ARY010 PAS staining kit Muto Pure Chemicals Cat# 15792 Experimental models: Cell lines Cryopreserved Hepatocytes Species:Human, Lot#S1412T, Lot#S1238 and Lot#S1350 KaLy-Cell Cat# HHCPC-2M hiPSC lines 201B7 CiRA Foundation N/A hiPSC lines 15M66 CiRA Foundation N/A MEF cells ReproCELL Inc. Cat# RCHEFC003 Oligonucleotides oligonucleotides sequences were listed in Table S1 – – Software and algorithms US National Library of Medicine National Institutes of Health website National Institutes of Health (NIH) RRID:SCR_011446 https://www.ncbi.nlm.nih. gov/pubmed/ Primer 3 Plus application Primer 3 Plus RRID:SCR_003081 http://www.bioinformatics. nl/cgi-bin/primer3plus/pimer 3 plus.cgi R software program R Development Core Team RRID:SCR_001905 https://www.r-project.org (Continued on next page) 16 iScience 25, 105052, October 21, 2022

    Cell Culture:

    Article Title: Induced hepatic stem cells are suitable for human hepatocyte production.
    Article Snippet: Penicillin-Streptomycin Solution (3100) FUJIFILM Wako Pure Chemical Corporation Cat# 16823191 ICG Sigma-Aldrich Cat# 155020 D-GaIN FUJIFILM Wako Pure Chemical Corporation Cat# 07505013 EzRIPA Lysis kit ATTO Cat# WSE-7420 EzApply ATTO Cat# 2332330 EzStandard PrestainBlue ATTO Cat# 2332347 c-PAGEL 10% ATTO Cat# 2331955 EzRun ATTO Cat# 2332310 P plus membranes ATTO Cat# 2322451 Filter paper ATTO Cat# 2392393 EzFastBlot ATTO Cat# 2332590 EzBlock Chemi ATTO Cat# 2332615 EzTBS ATTO Cat# 2332625 EzWestBlue ATTO Cat# 2332456 Precision Plus Protein All Blue Prestained Protein Standards Bio-Rad Laboratories, Inc. Cat# 1610373 Hanks’ Balanced Salt Solution (HBSS) Life Technologies Cat# 14025092 Critical commercial assays RNeasy Mini kit Qiagen N.V. Cat# 74106 SuperPREP II Cell Lysis & RT Kit for quantitative PCR TOYOBO CO., LTD. Cat# SCQ-401 FastStart Essential DNA Green Master Roche Cat# 06402712001 Luna Universal qPCR Master Mix New England Biolabs Inc. Cat# M3003E TaqMan Array 96-Well FAST Plate(Human Stem Cell Pluripotency) Applied Biosystems Cat# 4418722 TaqMan Fast Advanced Master Mix Thermo Fisher Scientific Cat# 4444963 Proteome Profiler Human Pluripotent Stem Cell Array Kit R&D Systems Cat# ARY010 PAS staining kit Muto Pure Chemicals Cat# 15792 Experimental models: Cell lines Cryopreserved Hepatocytes Species:Human, Lot#S1412T, Lot#S1238 and Lot#S1350 KaLy-Cell Cat# HHCPC-2M hiPSC lines 201B7 CiRA Foundation N/A hiPSC lines 15M66 CiRA Foundation N/A MEF cells ReproCELL Inc. Cat# RCHEFC003 Oligonucleotides oligonucleotides sequences were listed in Table S1 – – Software and algorithms US National Library of Medicine National Institutes of Health website National Institutes of Health (NIH) RRID:SCR_011446 https://www.ncbi.nlm.nih. gov/pubmed/ Primer 3 Plus application Primer 3 Plus RRID:SCR_003081 http://www.bioinformatics. nl/cgi-bin/primer3plus/pimer 3 plus.cgi R software program R Development Core Team RRID:SCR_001905 https://www.r-project.org (Continued on next page) 16 iScience 25, 105052, October 21, 2022



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